The Ghana Health Service has distributed HemoCue® point-of-care devices to measure hemoglobin concentration at the health facility level to assess the prevalence of anemia. The objective of this manual is to define and explain anemia, its causes and effects, and methods for diagnosis. It also outlines the procedure for measuring hemoglobin concentration with the HemoCue Hb 301 device. The target audience for this manual is staff from health facilities who have been selected to become master trainers. These selected personnel will travel to the districts to provide training to the health staff in the subcenters and community-based health planning and services zones.